Expert in culture of mammalian cell lines and primary tissue, transient and stable transfection, viability and apoptosis assays, ELISA, extraction of RNA/protein/DNA, real-time qPCR, genotyping, western blotting and gel electrophoresis. Experienced in tissue harvesting, immunohistochemistry, immunocytochemistry and transmission electron microscopy.
Main research areas
My principal research interests lie in the field of cell and molecular biology of the insulin-producing β-cells. My key areas of expertise are in vitro and ex vivo models of β-cell dysfunction and apoptosis in relation to type 1 diabetes. My research focuses on understanding the role of the type 1 diabetes candidates genes in relation to β-cell function and type 1 diabetes development.
(My current research focuses on unraveling the functions of the cathepsin protease family in the β-cells and their potential involvement in the pathogenesis of type 1 diabetes.)